E02C2754 Rat chenodeoxycholic acid ELISA kit
The Rat chenodeoxycholic acid ELISA kit can be used to identify samples from the rat species. chenodeoxycholic acid can also be called 3α,7α-Dihydroxy-5β-cholan-24-oic acid;chenodesoxycholic acid, chenocholic acid, CDCA.
Product Information | |
Cat. No. | E02C2754 |
Product Name | Rat chenodeoxycholic acid ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CDCA ELISA kit uses an anti-CDCA antibody and an CDCA-HRP conjugate in a competitive enzyme immunoassay method. CDCA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CDCA antibody binding site between CDCA from samples and CDCA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CDCA. Since the number of sites is limited, as more sites are occupied by CDCA from the sample, fewer sites are left to bind CDCA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDCA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CDCA and analogues was observed. | |
E02C2754 has been referenced in the below publications：
Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury,inflammation, and cholestasis in bile duct-ligated rats.
Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.
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