E02C0745 Rat Cholesterol ELISA ELISA
The Rat Cholesterol ELISA ELISA can be used to identify samples from the rat species. Cholesterol can also be called Cholesterin, Cholesteryl alcohol, CH.
Specifications of Rat Cholesterol ELISA ELISA
Product Information | |
Cat. No. | E02C0745 |
Product Name | Rat Cholesterol ELISA ELISA |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 mmol/L |
Sensitivity | 0.1 mmoL/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mmol/L | 1 vial |
STANDARD B (0.5mL) | 1.0 mmol/L | 1 vial |
STANDARD C (0.5mL) | 2.5 mmol/L | 1 vial |
STANDARD D (0.5mL) | 5.0 mmol/L | 1 vial |
STANDARD E (0.5mL) | 10 mmol/L | 1 vial |
STANDARD F (0.5mL) | 25 mmol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CH ELISA kit uses an anti-CH antibody and an CH-HRP conjugate in a competitive enzyme immunoassay method. CH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CH antibody binding site between CH from samples and CH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CH. Since the number of sites is limited, as more sites are occupied by CH from the sample, fewer sites are left to bind CH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CH concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Cholesterol ELISA ELISA
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Cholesterol ELISA ELISA
Citations of Rat Cholesterol ELISA ELISA
E02C0745 has been referenced in the below publications：
Screening and Optimization of Hyperlipidemia Rat Model of Several Kinds of Modeling Methods.
