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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Collagen Type Ⅲ ELISA kit (E02C0017)

E02C0017 Rat Collagen Type Ⅲ ELISA kit

The Rat Collagen Type Ⅲ ELISA kit can be used to identify samples from the rat species. Interleukin can also be called COL3A1, Collagen alpha 1(III) chain, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, COLIII.

Products

Specifications of Rat Collagen Type Ⅲ ELISA kit

Product Information

Cat. No.

E02C0017

Product Name

Rat Collagen Type Ⅲ ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

COLIII ELISA kit uses an anti-COLIII antibody and an COLIII-HRP conjugate in a competitive enzyme immunoassay method. COLIII-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-COLIII antibody binding site between COLIII from samples and COLIII-HRP conjugate, the intensity of the color is inversely proportional to the concentration of COLIII. Since the number of sites is limited, as more sites are occupied by COLIII from the sample, fewer sites are left to bind COLIII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COLIII concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Collagen Type Ⅲ ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between COLIII and analogues was observed.


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Summary of the Assay Procedure for Rat Collagen Type Ⅲ ELISA kit

Summary of the Assay Procedure for Rat Collagen Type Ⅲ ELISA kit

Citations of Rat Collagen Type Ⅲ ELISA kit

E02C0017 has been referenced in the below publications:

ShRNA targeted to rat Smad3 decreases the profibrosis role of TGFβ1 in HSC-T6 cells.

The effect of TGF- β1 on cell proliferation,cell cycle regulation and collagen expression in HSC-T6 cells.

Effect and mechanism of paeoniflorin on cell proliferation and collagen production induced by isoproterenol in cardiac fibroblasts.

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