E02C0014 Rat Collagen Type I ELISA kit
The Rat Collagen Type I ELISA kit can be used to identify samples from the rat species. Collagen Type I can also be called Collagen type I, Alpha 1 type I collagen, COL1A1, Collagen I alpha 1 polypeptide, Collagen Of Skin Tendon And Bone, Collagen Type 1, Collagen type I alpha 1, Pro alpha 1(I) collagen, Type I procollagen, Collagen alpha-1(II) chain, Collagen alpha-1(II) chain, Chondrocalcin, collagen alpha-1(I) chain preproprotein, COL1.
Product Information | |
Cat. No. | E02C0014 |
Product Name | Rat Collagen Type I ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COL1 ELISA kit uses an anti-ICOL1 antibody and an ICOL1-HRP conjugate in a competitive enzyme immunoassay method. ICOL1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ICOL1 antibody binding site between ICOL1 from samples and ICOL1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ICOL1. Since the number of sites is limited, as more sites are occupied by ICOL1 from the sample, fewer sites are left to bind ICOL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ICOL1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COL1 and analogues was observed. | |
E02C0014 has been referenced in the below publications：
Effect and Mechanism of Astragalus Total Flavonoids on Proliferation, Differentiation and Mineralization of Rat Primary Osteoblast.
Research on the Mechanism of SAL on Regulating Phenotypic Expression of Rat Calvarial Osteoblasts.
ShRNA targeted to rat Smad3 decreases the profibrosis role of TGFβ1 in HSC-T6 cells.
The effect of TGF- β1 on cell proliferation，cell cycle regulation and collagen expression in HSC-T6 cells.
Effect and mechanism of paeoniflorin on cell proliferation and collagen production induced by isoproterenol in cardiac fibroblasts.
Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phenylboroinc Acid Based Low-molecular-weight Gelators.
Ameliorative effect of ursolic acid on renal fibrosis in adenine-induced chronic kidney disease in rats.
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