E02C0779 Rat Collagen Type II ELISA kit
The Rat Collagen Type II ELISA kit can be used to identify samples from the rat species. Collagen Type II can also be called Alpha-1 type II collagen;COL2A1, Col Ⅱ.
Specifications of Rat Collagen Type II ELISA kit
Product Information | |
Cat. No. | E02C0779 |
Product Name | Rat Collagen Type II ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Col Ⅱ ELISA kit uses an anti-Col Ⅱ antibody and an Col Ⅱ-HRP conjugate in a competitive enzyme immunoassay method. Col Ⅱ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Col Ⅱ antibody binding site between Col Ⅱ from samples and Col Ⅱ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Col Ⅱ. Since the number of sites is limited, as more sites are occupied by Col Ⅱ from the sample, fewer sites are left to bind Col Ⅱ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Col Ⅱ concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Collagen Type II ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Col Ⅱ and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Collagen Type II ELISA kit
Citations of Rat Collagen Type II ELISA kit
E02C0779 has been referenced in the below publications：
Control of MSC Differentiation by Tuning the Alkyl Chain Length of Phenylboroinc Acid Based Low-molecular-weight Gelators.
