E02C0047 Rat Collagen Type IV ELISA kit
The Rat Collagen Type IV ELISA kit can be used to identify samples from the rat species. Collagen Type IV can also be called ColIV, Col4, COL4.
Specifications of Rat Collagen Type IV ELISA kit
Product Information | |
Cat. No. | E02C0047 |
Product Name | Rat Collagen Type IV ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 10 ng/ml | 1 vial |
STANDARD C (0.5mL) | 25 ng/ml | 1 vial |
STANDARD D (0.5mL) | 50 ng/ml | 1 vial |
STANDARD E (0.5mL) | 100 ng/ml | 1 vial |
STANDARD F (0.5mL) | 250 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COL4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for COL4. Standards or samples are then added to the microtiter plate wells and COL4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of COL4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for COL4 are added to each well to “sandwich” the COL4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain COL4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Collagen Type IV ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COL4 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Collagen Type IV ELISA kit
Citations of Rat Collagen Type IV ELISA kit
E02C0047 has been referenced in the below publications：
Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats.
