E02C0051 Rat Collagenase I ELISA kit
The Rat Collagenase I ELISA kit can be used to identify samples from the rat species. Collagenase I can also be called Microbial collagenase, matrix metalloproteinase 1, Collagenase I.
Specifications of Rat Collagenase I ELISA kit
Product Information | |
Cat. No. | E02C0051 |
Product Name | Rat Collagenase I ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Collagenase I ELISA kit uses an anti-Collagenase I antibody and an Collagenase I-HRP conjugate in a competitive enzyme immunoassay method. Collagenase I-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Collagenase I antibody binding site between Collagenase I from samples and Collagenase I-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Collagenase I. Since the number of sites is limited, as more sites are occupied by Collagenase I from the sample, fewer sites are left to bind Collagenase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Collagenase I concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Collagenase I ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Collagenase I and analogues was observed. | |
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Summary of the Assay Procedure for Rat Collagenase I ELISA kit
Citations of Rat Collagenase I ELISA kit
E02C0051 has been referenced in the below publications：
Study of the mechanism of lipopolysaccharide on the expression of connective tissue growth factor in primary hepatic stellate cells of rats.
