E02C0085 Rat Creatine Kinase MB isoenzyme ELISA kit
The Rat Creatine Kinase MB isoenzyme ELISA kit can be used to identify samples from the rat species. Creatine Kinase MB isoenzyme can also be called CK-MB, Creatine Kinase MB isoenzyme.
Specifications of Rat Creatine Kinase MB isoenzyme ELISA kit
Product Information | |
Cat. No. | E02C0085 |
Product Name | Rat Creatine Kinase MB isoenzyme ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CKMB ELISA kit uses an anti-CKMB antibody and an CKMB-HRP conjugate in a competitive enzyme immunoassay method. CKMB-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CKMB antibody binding site between CKMB from samples and CKMB-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CKMB. Since the number of sites is limited, as more sites are occupied by CKMB from the sample, fewer sites are left to bind CKMB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKMB concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Creatine Kinase MB isoenzyme ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CKMB and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Creatine Kinase MB isoenzyme ELISA kit
Citations of Rat Creatine Kinase MB isoenzyme ELISA kit
E02C0085 has been referenced in the below publications：
Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation.
Protective Effect of Bisoprolol Preconditioning on Myocardial Ischemia Reperfusion Injury in Rats by the Medium of PI3K/AKT/GS3K-b.
Experimental Study On Cardioprotection and Mechanism Of Combined Limb Remote Ischemic Postconditioning and Sevoflurane postcondmoning Against Rat Myocardial Ischemia-Reperfusion Injury and Its mechanism.
Protection effect of Yindan Xinnaotong capsule and main compositions compatibility on myocardial ischemia/reperfusion injury.
The experimental and clinical research on Protective Effect and Mechanism of Sofren on Myocardial Ischemia reperfusion Injury.
Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway.
