E02C0400 Rat Creatine Kinase, Muscle ELISA kit
The Rat Creatine Kinase, Muscle ELISA kit can be used to identify samples from the rat species. Creatine Kinase, Muscle can also be called CKMM, CPK-M, M-CK.
Specifications of Rat Creatine Kinase, Muscle ELISA kit
Product Information | |
Cat. No. | E02C0400 |
Product Name | Rat Creatine Kinase, Muscle ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CK MM ELISA kit uses an anti-CK MM antibody and an CK MM-HRP conjugate in a competitive enzyme immunoassay method. CK MM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CK MM antibody binding site between CK MM from samples and CK MM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CK MM. Since the number of sites is limited, as more sites are occupied by CK MM from the sample, fewer sites are left to bind CK MM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CK MM concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Creatine Kinase, Muscle ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CK MM and analogues was observed. | |
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Summary of the Assay Procedure for Rat Creatine Kinase, Muscle ELISA kit
Citations of Rat Creatine Kinase, Muscle ELISA kit
E02C0400 has been referenced in the below publications：
Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation.
