E02C0071 Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit
The Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit can be used to identify samples from the rat species. Cross Linked C Telopeptide Of Type II Collagen can also be called Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,Cross Linked C-Telopeptide Of Type II Collagen ,CTX-Ⅱ.
Product Information | |
Cat. No. | E02C0071 |
Product Name | Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CTx2 ELISA kit uses an anti-CTx2 antibody and an CTx2-HRP conjugate in a competitive enzyme immunoassay method. CTx2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTx2 antibody binding site between CTx2 from samples and CTx2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTx2. Since the number of sites is limited, as more sites are occupied by CTx2 from the sample, fewer sites are left to bind CTx2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTx2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CTx2 and analogues was observed. | |
E02C0071 has been referenced in the below publications：
The effects of immobilization and PTH on the capacity of spontaneous repair in an articular cartilage defect animal model.
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