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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit (E02C0071)

E02C0071 Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

The Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit can be used to identify samples from the rat species. Cross Linked C Telopeptide Of Type II Collagen can also be called Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,Cross Linked C-Telopeptide Of Type II Collagen ,CTX-Ⅱ.

Products

Specifications of Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Product Information

Cat. No.

E02C0071

Product Name

Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CTx2 ELISA kit uses an anti-CTx2 antibody and an CTx2-HRP conjugate in a competitive enzyme immunoassay method. CTx2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTx2 antibody binding site between CTx2 from samples and CTx2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTx2. Since the number of sites is limited, as more sites are occupied by CTx2 from the sample, fewer sites are left to bind CTx2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTx2 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CTx2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Summary of the Assay Procedure for Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

Citations of Rat Cross Linked C Telopeptide Of Type II Collagen ELISA kit

E02C0071 has been referenced in the below publications:

The effects of immobilization and PTH on the capacity of spontaneous repair in an articular cartilage defect animal model.

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