E02C0754 Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit
The Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit can be used to identify samples from the rat species. Cross linked C terminal Telopeptide of type I Collagen can also be called CTX-I, CTX1.
Product Information | |
Cat. No. | E02C0754 |
Product Name | Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CTX1 ELISA kit uses an anti-CTX1 antibody and an CTX1-HRP conjugate in a competitive enzyme immunoassay method. CTX1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTX1 antibody binding site between CTX1 from samples and CTX1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTX1. Since the number of sites is limited, as more sites are occupied by CTX1 from the sample, fewer sites are left to bind CTX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTX1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CTX1 and analogues was observed. | |
E02C0754 has been referenced in the below publications：
The effects of estrogen and PTH on spinal Wnt/β-catenin pathway and degeneration in ovariectomized rats.
Effects of hepcidin on bone metabolism in ovariectomized rats.
Estrogen alone or in combination with parathyroid hormone can decrease vertebral MEF2 and sclerostin expression and increase vertebral bone mass in ovariectomized rats.
The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats.
Involvement of periostin-sclerostin-Wnt/β-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin.
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