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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit (E02C0754)

  • Cell Biology

E02C0754 Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

The Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit can be used to identify samples from the rat species. Cross linked C terminal Telopeptide of type I Collagen can also be called CTX-I, CTX1.

Products

Specifications of Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

Product Information

Cat. No.

E02C0754

Product Name

Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CTX1 ELISA kit uses an anti-CTX1 antibody and an CTX1-HRP conjugate in a competitive enzyme immunoassay method. CTX1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CTX1 antibody binding site between CTX1 from samples and CTX1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CTX1. Since the number of sites is limited, as more sites are occupied by CTX1 from the sample, fewer sites are left to bind CTX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTX1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CTX1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

Summary of the Assay Procedure for Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

Citations of Rat Cross linked C terminal Telopeptide of type I Collagen ELISA kit

E02C0754 has been referenced in the below publications:

The effects of estrogen and PTH on spinal Wnt/β-catenin pathway and degeneration in ovariectomized rats.

Effects of hepcidin on bone metabolism in ovariectomized rats.

Estrogen alone or in combination with parathyroid hormone can decrease vertebral MEF2 and sclerostin expression and increase vertebral bone mass in ovariectomized rats.

The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar rats.

Involvement of periostin-sclerostin-Wnt/β-catenin signaling pathway in the prevention of neurectomy-induced bone loss by naringin.


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