E02G0003 Rat Cyclic Guanosine Monophosphate ELISA kit
The Rat Cyclic Guanosine Monophosphate ELISA kit can be used to identify samples from the rat species. Cyclic Guanosine Monophosphate can also be called Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate, cGMP.
Product Information | |
Cat. No. | E02G0003 |
Product Name | Rat Cyclic Guanosine Monophosphate ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 pmol/mL |
Sensitivity | 1.0 pmol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pmol/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 pmol/mL | 1 vial |
STANDARD C (0.5mL) | 10 pmol/mL | 1 vial |
STANDARD D (0.5mL) | 25 pmol/mL | 1 vial |
STANDARD E (0.5mL) | 50 pmol/mL | 1 vial |
STANDARD F (0.5mL) | 100 pmol/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
cGMP ELISA kit uses an anti-cGMP antibody and an cGMP-HRP conjugate in a competitive enzyme immunoassay method. cGMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cGMP antibody binding site between cGMP from samples and cGMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cGMP. Since the number of sites is limited, as more sites are occupied by cGMP from the sample, fewer sites are left to bind cGMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cGMP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between cGMP and analogues was observed. | |
E02G0003 has been referenced in the below publications：
Design and Synthesis of a small peptide RWR containing RGD and initial exploration of function.
Vasodilatory effect of formaldehyde via the NO/cGMP pathway and the regulation of expression of KATP, BKCa and L-type Ca2+ channels.
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