E02C0834 Rat Cystathionine Gamma Lyase ELISA kit
The Rat Cystathionine Gamma Lyase ELISA kit can be used to identify samples from the rat species. Cystathionine Gamma Lyase can also be called CTH, Cystathionase; Cysteine-protein sulfhydrase; Gamma-cystathionase.
Product Information | |
Cat. No. | E02C0834 |
Product Name | Rat Cystathionine Gamma Lyase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CSE ELISA kit uses an anti-CSE antibody and an CSE-HRP conjugate in a competitive enzyme immunoassay method. CSE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CSE antibody binding site between CSE from samples and CSE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CSE. Since the number of sites is limited, as more sites are occupied by CSE from the sample, fewer sites are left to bind CSE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSE concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CSE and analogues was observed. | |
E02C0834 has been referenced in the below publications：
Protective Effects of Allicin on Acute Myocardial Infarction in Rats via Hydrogen Sulfide-mediated Regulation of Cororary Arterial Vasomotor Functior and Myocardial Calcium Transport.
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