E02C0873 Rat Cytochrome-C ELISA
The Rat Cytochrome-C ELISA can be used to identify samples from the rat species. Cytochrome-C can also be called CYCS, CYC, HCS, THC4, cytochrome c, somatic, Cytochrome c, cyt c
Specifications of Rat Cytochrome-C ELISA kit
Product Information | |
Cat. No. | E02C0873 |
Product Name | Rat Cytochrome-C ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Cyt-C ELISA kit uses an anti-Cyt-C antibody and an Cyt-C-HRP conjugate in a competitive enzyme immunoassay method. Cyt-C-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Cyt-C antibody binding site between Cyt-C from samples and Cyt-C-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Cyt-C. Since the number of sites is limited, as more sites are occupied by Cyt-C from the sample, fewer sites are left to bind Cyt-C-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Cyt-C concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Cytochrome-C ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Cyt-C and analogues was observed. | |
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Summary of the Assay Procedure for Rat Cytochrome-C ELISA kit
Citations of Rat Cytochrome-C ELISA kit
E02C0873 has been referenced in the below publications：
The study on the effects of quercetin,vitamin E and amifostine on Cisplatin-induced ovarian damage in famale rats.
Exploration of the optimal dose of HOE-642 for the protection of neuronal mitochondrial function after cardiac arrest in rats.
