E02C0016 Rat Cytochrome c oxidase subunit II ELISA kit
The Rat Cytochrome c oxidase subunit II ELISA kitcan be used to identify samples from the rat species. Cytochrome c oxidase subunit II can also be called MTCO2, MT-CO2, COII, COXII, Mitochondrially Encoded Cytochrome C Oxidase II, Cytochrome c oxidase polypeptide II.
Product Information | |
Cat. No. | E02C0016 |
Product Name | Rat Cytochrome c oxidase subunit II ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
COX2 ELISA kit uses an anti-COX2 antibody and an COX2-HRP conjugate in a competitive enzyme immunoassay method. COX2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-COX2 antibody binding site between COX2 from samples and COX2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of COX2. Since the number of sites is limited, as more sites are occupied by COX2 from the sample, fewer sites are left to bind COX2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COX2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between COX2 and analogues was observed. | |
E02C0016 has been referenced in the below publications：
Protective effect of Averrhoa bilimbi L. fruit extract on ulcerative colitis in wistar rats via regulation of inflammatory mediators and cytokines.
Intrahepatic transplantation of adipose‑derived stem cells attenuates the progression of non‑alcoholic fatty liver disease in rats.
Related Bluegene Biotech Products
BlueGene Biotech News
