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BlueGene Biotech Rat Cytochrome P450 ELISA kit (E02C0863)

E02C0863 Rat Cytochrome P450 ELISA kit

The Rat Cytochrome P450 ELISA kit can be used to identify samples from the rat species. Cytochrome P450 can also be called CYP450, P450, CYP.

Products

Specifications of Rat Cytochrome P450 ELISA kit

Product Information

Cat. No.

E02C0863

Product Name

Rat Cytochrome P450 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml

1 vial

STANDARD B (0.5mL)

100 pg/ml

1 vial

STANDARD C (0.5mL)

250 pg/ml

1 vial

STANDARD D (0.5mL)

500 pg/ml

1 vial

STANDARD E (0.5mL)

1000 pg/ml

1 vial

STANDARD F (0.5mL)

2500 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CYP450 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP450. Standards or samples are then added to the microtiter plate wells and CYP450 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP450 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP450 are added to each well to “sandwich” the CYP450 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP450 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP450 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cytochrome P450 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CYP450 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Cytochrome P450 ELISA kit

Summary of the Assay Procedure for Rat Cytochrome P450 ELISA kit

Citations of Rat Cytochrome P450 ELISA kit

E02C0863 has been referenced in the below publications:

Influences of Cigu (Sagittaria Sagittifolia) on cytochrome P 450 in rats with liver damage induced by isoniazid and rifampin.

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