E02C0863 Rat Cytochrome P450 ELISA kit
The Rat Cytochrome P450 ELISA kit can be used to identify samples from the rat species. Cytochrome P450 can also be called CYP450, P450, CYP.
Specifications of Rat Cytochrome P450 ELISA kit
Product Information | |
Cat. No. | E02C0863 |
Product Name | Rat Cytochrome P450 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/ml | 1 vial |
STANDARD B (0.5mL) | 100 pg/ml | 1 vial |
STANDARD C (0.5mL) | 250 pg/ml | 1 vial |
STANDARD D (0.5mL) | 500 pg/ml | 1 vial |
STANDARD E (0.5mL) | 1000 pg/ml | 1 vial |
STANDARD F (0.5mL) | 2500 pg/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CYP450 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP450. Standards or samples are then added to the microtiter plate wells and CYP450 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP450 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP450 are added to each well to “sandwich” the CYP450 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP450 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP450 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Cytochrome P450 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CYP450 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Cytochrome P450 ELISA kit
Citations of Rat Cytochrome P450 ELISA kit
E02C0863 has been referenced in the below publications：
Influences of Cigu (Sagittaria Sagittifolia) on cytochrome P 450 in rats with liver damage induced by isoniazid and rifampin.
