Rat CytokeRatin Fragment Antigen 21.1 ELISA kit (E02C0010)

BlueGene Biotech Rat CytokeRatin Fragment Antigen 21.1 ELISA kit (E02C0010)

E02C0010 Rat CytokeRatin Fragment Antigen 21.1 ELISA kit

The Rat CytokeRatin Fragment Antigen 21.1 ELISA kit can be used to identify samples from the rat species. CytokeRatin Fragment Antigen 21.1 can also be called fragment of cytokeratin 19 (CK19), CYFRA21.1.

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Specifications of Rat CytokeRatin Fragment Antigen 21.1 ELISA kit

Product Information
Cat. No.	E02C0010
Product Name	Rat CytokeRatin Fragment Antigen 21.1 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

CYFRA21.1 ELISA kit uses an anti-CYFRA21.1 antibody and an CYFRA21.1-HRP conjugate in a competitive enzyme immunoassay method. CYFRA21.1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CYFRA21.1 antibody binding site between CYFRA21.1 from samples and CYFRA21.1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CYFRA21.1. Since the number of sites is limited, as more sites are occupied by CYFRA21.1 from the sample, fewer sites are left to bind CYFRA21.1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYFRA21.1 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat CytokeRatin Fragment Antigen 21.1 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between CYFRA21.1 and analogues was observed.