E02D0003 Rat Dehydroepiandrosterone ELISA kit
The Rat Dehydroepiandrosterone ELISA kit can be used to identify samples from the rat species. Dehydroepiandrosterone can also be called DHEA, androstenolone.
Specifications of Rat Dehydroepiandrosterone ELISA kit
Product Information | |
Cat. No. | E02D0003 |
Product Name | Rat Dehydroepiandrosterone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DHEA ELISA kit uses an anti-DHEA antibody and an DHEA-HRP conjugate in a competitive enzyme immunoassay method. DHEA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DHEA antibody binding site between DHEA from samples and DHEA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DHEA. Since the number of sites is limited, as more sites are occupied by DHEA from the sample, fewer sites are left to bind DHEA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHEA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Dehydroepiandrosterone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DHEA and analogues was observed. | |
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Summary of the Assay Procedure for Rat Dehydroepiandrosterone ELISA kit
Citations of Rat Dehydroepiandrosterone ELISA kit
E02D0003 has been referenced in the below publications：
The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.
