En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Dehydroepiandrosterone ELISA kit (E02D0003)

E02D0003 Rat Dehydroepiandrosterone ELISA kit

The Rat Dehydroepiandrosterone ELISA kit can be used to identify samples from the rat species. Dehydroepiandrosterone can also be called DHEA, androstenolone.

Products

Specifications of Rat Dehydroepiandrosterone ELISA kit

Product Information

Cat. No.

E02D0003

Product Name

Rat Dehydroepiandrosterone ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DHEA ELISA kit uses an anti-DHEA antibody and an DHEA-HRP conjugate in a competitive enzyme immunoassay method. DHEA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-DHEA antibody binding site between DHEA from samples and DHEA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of DHEA. Since the number of sites is limited, as more sites are occupied by DHEA from the sample, fewer sites are left to bind DHEA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHEA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Dehydroepiandrosterone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DHEA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Dehydroepiandrosterone ELISA kit

Summary of the Assay Procedure for Rat Dehydroepiandrosterone ELISA kit

Citations of Rat Dehydroepiandrosterone ELISA kit

E02D0003 has been referenced in the below publications:

The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.