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BlueGene Biotech Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit (E02D0012)

E02D0012 Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

The Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit can be used to identify samples from the rat species. Dimethylarginine Dimethylaminohydrolase 2 can also be called DDAH2.

Products

Specifications of Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

Product Information

Cat. No.

E02D0012

Product Name

Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DDAH2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DDAH2 antibody and an DDAH2-HRP conjugate. The assay sample and buffer are incubated together with DDAH2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDAH2 concentration since DDAH2 from samples and DDAH2-HRP conjugate compete for the anti-DDAH2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDAH2 from the sample, fewer sites are left to bind DDAH2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDAH2 concentration in each sample is interpolated from this standard curve. 


Quality Control on Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between DDAH2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

Summary of the Assay Procedure for Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

Citations of Rat Dimethylarginine Dimethylaminohydrolase 2 ELISA kit

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