E02E0023 Rat E2 estradiol ELISA kit
The Rat E2 estradiol ELISA kit can be used to identify samples from the rat species. Estradiol can also be called Estradiol, E2 estradiol, 17B-Estradiol, E2, Oestradiol, Beta-Estradiol, E2.
Specifications of Rat E2 estradiol ELISA kit
Product Information | |
Cat. No. | E02E0023 |
Product Name | Rat E2 estradiol ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
E2 ELISA kit uses an anti-E2 antibody and an E2-HRP conjugate in a competitive enzyme immunoassay method. E2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-E2 antibody binding site between E2 from samples and E2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of E2. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat E2 estradiol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between E2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat E2 estradiol ELISA kit
Citations of Rat E2 estradiol ELISA kit
E02E0023 has been referenced in the below publications：
The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.
益肾健脾通利活血方治疗痰湿型闭经理论与实验研究。
The study on the effects of quercetin, vitamin E and amifostine on Cisplatin-induced ovarian damage in famale rats.
EXPERIMENTAL STUDY ON BUSHENNING REGULATING RATNEUROTRANSMITTERS AND REPRODUCTIVE FUNCTION IN THE OVULATION INHIBITION OF KIDNEY YANG.
Effects of high exposure of endogenous reproductive hormones on perimenopausal and postmenopausal blood pressure in rats.
Studys on cryopreservation and autotransplantion of mice ovarian tissue.
The study on the effects of quercetin,vitamin E and amifostine on Cisplatin-induced ovarian damage in famale rats.
Protective Effect of Saponins from Bupleurum Chinense on Ethanol-induced Gastric Lesion.
Effects of the timing of estrogen replacement therapy on cognitive function of ovariectomized rats.
Establishment of in-vitro three dimensional rat follicle culture system and validation of the applicability as an in vitro female reproductive toxicity testing system.
