Rat Endothelial Nitric Oxide Synthase ELISA kit (E02N0024)

BlueGene Biotech Rat Endothelial Nitric Oxide Synthase ELISA kit (E02N0024)

E02N0024 Rat Endothelial Nitric Oxide Synthase ELISA kit

The Rat Endothelial Nitric Oxide Synthase ELISA kitcan be used to identify samples from the rat species. Endothelial Nitric Oxide Synthase can also be called NOS-3, cNOS, Constitutive NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III.

Products

Specifications of Rat Endothelial Nitric Oxide Synthase ELISA kit

Product Information
Cat. No.	E02N0024
Product Name	Rat Endothelial Nitric Oxide Synthase ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5.0 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

eNOS ELISA kit uses an anti-eNOS antibody and an eNOS-HRP conjugate in a competitive enzyme immunoassay method. eNOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-eNOS antibody binding site between eNOS from samples and eNOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of eNOS. Since the number of sites is limited, as more sites are occupied by eNOS from the sample, fewer sites are left to bind eNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Endothelial Nitric Oxide Synthase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between eNOS and analogues was observed.