E02L0300 Rat Fatty Acid Binding Protein 1, Liver ELISA Kit
The Rat Fatty Acid Binding Protein 1, Liver ELISA Kit can be used to identify samples from the rat species. Fatty Acid Binding Protein 1, Liver can also be called LFABP, FABP1, FABPL, L-FABP, fatty acid binding protein 1.
Product Information | |
Cat. No. | E02L0300 |
Product Name | Rat Fatty Acid Binding Protein 1, Liver ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FABP1 ELISA kit uses an anti-FABP1 antibody and an FABP1-HRP conjugate in a competitive enzyme immunoassay method. FABP1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FABP1 antibody binding site between FABP1 from samples and FABP1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FABP1. Since the number of sites is limited, as more sites are occupied by FABP1 from the sample, fewer sites are left to bind FABP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FABP1 and analogues was observed. | |
E02L0300 has been referenced in the below publications：
Value of serum liver fatty acid-binding protein in monitoring of hepatic function after the ischemiareperfusion injury.
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