En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Fibrinogen ELISA kit (E02F0007)

E02F0007 Rat Fibrinogen ELISA kit

The Rat Fibrinogen ELISA kit can be used to identify samples from the rat species. Fibrinogen can also be called F1, FI, Fbg, Coagulation Factor I, Fb.

Products

Specifications of Rat Fibrinogen ELISA kit

Product Information

Cat. No.

E02F0007

Product Name

Rat Fibrinogen ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Fb ELISA kit uses an anti-Fb antibody and an Fb-HRP conjugate in a competitive enzyme immunoassay method. Fb-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Fb antibody binding site between Fb from samples and Fb-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Fb. Since the number of sites is limited, as more sites are occupied by Fb from the sample, fewer sites are left to bind Fb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Fb concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Fibrinogen ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Fb and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Fibrinogen ELISA kit

Summary of the Assay Procedure for Rat Fibrinogen ELISA kit

Citations of Rat Fibrinogen ELISA kit

E02F0007 has been referenced in the below publications:

Study on the protective effects of Qishen Yiqi pill on myocardial ischemia reperfusion injury in rats.

Influence of Endothelial Function and Blood Rheology in Diabetic Rats by Paeonol and Tanshinol.

Pharmacokinetics, tissue distribution and excretion of porcine fibrinogen after intraperitoneal injection of a porcine-derived fibrin glue to rats.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.