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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Fibronectin ELISA kit (E02F0004)

E02F0004 Rat Fibronectin ELISA kit

The Rat Fibronectin ELISA kit can be used to identify samples from the rat species. Fibronectin can also be called CIG, Cold insoluble globulin, ED B, ED-B, fibronectin 1, FINC, FN 1 FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor, MSF, Transformation sensitive protein, Fibronectin, Cold-insoluble globulin.

Products

Specifications of Rat Fibronectin ELISA kit

Product Information

Cat. No.

E02F0004

Product Name

Rat Fibronectin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FN ELISA kit uses an anti-FN antibody and an FN-HRP conjugate in a competitive enzyme immunoassay method. FN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FN antibody binding site between FN from samples and FN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FN. Since the number of sites is limited, as more sites are occupied by FN from the sample, fewer sites are left to bind FN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FN concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Fibronectin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Fibronectin ELISA kit

Summary of the Assay Procedure for Rat Fibronectin ELISA kit

Citations of Rat Fibronectin ELISA kit


E02F0004 has been referenced in the below publications:

Effects of Sparganii Pill Treating Intestinal Fibrosis in Rats of Crohn's Disease.

Ameliorative effect of ursolic acid on renal fibrosis in adenine-induced chronic kidney disease in rats.

Curcumin Suppresses Intestinal Fibrosis by Inhibition of PPARr-Mediated Epithelial-Mesenchymal Transition.

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