E02F0001 Rat Follicle Stimulating Hormone ELISA kit
The Rat Follicle Stimulating Hormone ELISA kit can be used to identify samples from the rat species. Follicle Stimulating Hormone can also be called Follicle Stimulating Hormone, Follicle stimulating hormone beta polypeptide, Follicle stimulating hormone beta subunit, Follitropin beta chain, Follitropin beta chain precursor, Follitropin subunit beta, FSH B, FSH-B, FSH-beta, FSHB, FSHbeta, FSH.
Product Information | |
Cat. No. | E02F0001 |
Product Name | Rat Follicle Stimulating Hormone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 10 ng/ml | 1 vial |
STANDARD D (0.5mL) | 25 ng/ml | 1 vial |
STANDARD E (0.5mL) | 50 ng/ml | 1 vial |
STANDARD F (0.5mL) | 100 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FSH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FSH. Standards or samples are then added to the microtiter plate wells and FSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FSH are added to each well to “sandwich” the FSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSH concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FSH and analogues was observed. | |
E02F0001 has been referenced in the below publications：
The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.
Influences of Maca and Tongkat Ali on The Serum Testosterone Level of Swimming Training Rats.
以肝脾为核也干预HPRL模型大鼠下丘脑多己胺腺昔酸环化酶通路机制的探索。
EXPERIMENTAL STUDY ON BUSHENNING REGULATING RATNEUROTRANSMITTERS AND REPRODUCTIVE FUNCTION IN THE OVULATION INHIBITION OF KIDNEY YANG.
The study on the effects of quercetin,vitamin E and amifostine on Cisplatin-induced ovarian damage in famale rats.
东革阿里对游泳训练大鼠血睾酮水平及运动能力的影响。
Effects of the timing of estrogen replacement therapy on cognitive function of ovariectomized rats.
Effect of Melatonin Treatment during Pregnancy on Growth of Female Offspring in Rat.
参麦黄连阿胶汤治疗绝经综合征的药效学实验。
Quantitative changes in testicular structure and function in rat exposed to mobile phone radiation.
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