E02F0002 Rat Free Fatty Acid ELISA kit
The Rat Free Fatty Acid ELISA kit can be used to identify samples from the rat species. Free Fatty Acid can also be called NEFA,nonestesterified fatty acid；free fatty acid, FFA.
Specifications of Rat Free Fatty Acid ELISA kit
Product Information | |
Cat. No. | E02F0002 |
Product Name | Rat Free Fatty Acid ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD F (0.5mL) | 10 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FFA ELISA kit uses an anti-FFA antibody and an FFA-HRP conjugate in a competitive enzyme immunoassay method. FFA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FFA antibody binding site between FFA from samples and FFA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FFA. Since the number of sites is limited, as more sites are occupied by FFA from the sample, fewer sites are left to bind FFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FFA concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Free Fatty Acid ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FFA and analogues was observed. | |
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Summary of the Assay Procedure for Rat Free Fatty Acid ELISA kit
Citations of Rat Free Fatty Acid ELISA kit
E02F0002 has been referenced in the below publications：
Effects of curcumin on nonalcoholic steatohepatitis in rats and its mechanisms.
Chinese medicine Jinlida ameliorates high-fat dietinduced insulin resistance in rats through alleviating lipid accumulation in skeletal muscle.
The Effect of Acupuncture on Insulin-resistance in Obese Rats.
Liver injury attenuation by curcumin in a rat NASH model: an Nrf2 activation-mediated effect?.
