Rat G protein-coupled receptor kinase 2 ELISA kit (E02G0463)

BlueGene Biotech Rat G protein-coupled receptor kinase 2 ELISA kit (E02G0463)

E02G0463 Rat G protein-coupled receptor kinase 2 ELISA kit

The Rat G protein-coupled receptor kinase 2 ELISA kit can be used to identify samples from the rat species. G protein-coupled receptor kinase 2 can also be called GRK2, BARK1, BETA, ARK1, ADRBK1, G protein-coupled receptor kinase 2, Beta-adrenergic receptor kinase.

Products

Specifications of Rat G protein-coupled receptor kinase 2 ELISA kit

Product Information
Cat. No.	E02G0463
Product Name	Rat G protein-coupled receptor kinase 2 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	0.5-10 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

GRK2 ELISA kit uses an anti-GRK2 antibody and an GRK2-HRP conjugate in a competitive enzyme immunoassay method. GRK2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GRK2 antibody binding site between GRK2 from samples and GRK2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GRK2. Since the number of sites is limited, as more sites are occupied by GRK2 from the sample, fewer sites are left to bind GRK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GRK2 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat G protein-coupled receptor kinase 2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between GRK2 and analogues was observed.