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BlueGene Biotech Rat Gastric Inhibitory Polypeptide ELISA kit (E02G0177)

E02G0177 Rat Gastric Inhibitory Polypeptide ELISA kit

The Rat Gastric Inhibitory Polypeptide ELISA kit can be used to identify samples from the rat species. Gastric Inhibitory Polypeptide can also be called Gastric Inhibitory polypeptide, Gastric inhibitory polypeptide precursor, Glucose dependent insulinotropic polypeptide, Gastric Inhibitory Peptide, Glucose-dependent insulinotropic polypeptide, Incretin hormone, GIP.

Products

Specifications of Rat Gastric Inhibitory Polypeptide ELISA kit

Product Information

Cat. No.

E02G0177

Product Name

Rat Gastric Inhibitory Polypeptide ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GIP ELISA kit uses an anti-GIP antibody and an GIP-HRP conjugate in a competitive enzyme immunoassay method. GIP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GIP antibody binding site between GIP from samples and GIP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GIP. Since the number of sites is limited, as more sites are occupied by GIP from the sample, fewer sites are left to bind GIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GIP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Gastric Inhibitory Polypeptide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GIP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Gastric Inhibitory Polypeptide ELISA kit

Summary of the Assay Procedure for Rat Gastric Inhibitory Polypeptide ELISA kit

Citations of Rat Gastric Inhibitory Polypeptide ELISA kit

E02G0177 has been referenced in the below publications:

INFLUENCE OF GASTRIC BYPASS AND SLEEVE GASTRECTOMY ON BLOOD GLUCOSE AND SERUM GIP、GHRELIN OF NORMAL WISTAR RATS.

Clinical Observation and Mechanism of Spleen Qi Treatment of Spleen Deficiency Type 2 Diabetes.

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