E02G0281 Rat Gastrin ELISA kit
The Rat Gastrin ELISA kit can be used to identify samples from the rat species. Gastrin can also be called GAST, GAS, gastrin, GT.
Specifications of Rat Gastrin ELISA kit
Product Information | |
Cat. No. | E02G0281 |
Product Name | Rat Gastrin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GT ELISA kit uses an anti-GT antibody and an GT-HRP conjugate in a competitive enzyme immunoassay method. GT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GT antibody binding site between GT from samples and GT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GT. Since the number of sites is limited, as more sites are occupied by GT from the sample, fewer sites are left to bind GT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GT concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Gastrin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Gastrin ELISA kit
Citations of Rat Gastrin ELISA kit
E02G0281 has been referenced in the below publications：
Clinical Observation and Mechanism of Spleen Qi Treatment of Spleen Deficiency Type 2 Diabetes.
短期和长期的埃索美拉唑对SD大鼠胃黏膜的影响。
