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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Ghrelin ELISA kit (E02G0013)

E02G0013 Rat Ghrelin ELISA kit

The Rat Ghrelin ELISA kit can be used to identify samples from the rat species. Ghrelin can also be called Appetite regulating hormone, Ghrelin 27, Ghrelin 28, Ghrelin/obestatin prepropeptide, Growth hormone releasing peptide, Growth hormone secretagogue, M46 protein, Motilin related peptide, MTLRP, Obestatin, Obestatin preprohormone, Ghrelin.

Products

Specifications of Rat Ghrelin ELISA kit

Product Information

Cat. No.

E02G0013

Product Name

Rat Ghrelin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Ghrelin ELISA kit uses an anti-Ghrelin antibody and an Ghrelin-HRP conjugate in a competitive enzyme immunoassay method. Ghrelin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Ghrelin antibody binding site between Ghrelin from samples and Ghrelin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Ghrelin. Since the number of sites is limited, as more sites are occupied by Ghrelin from the sample, fewer sites are left to bind Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ghrelin concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Ghrelin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Ghrelin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Ghrelin ELISA kit

Summary of the Assay Procedure for Rat Ghrelin ELISA kit

Citations of Rat Ghrelin ELISA kit

E02G0013 has been referenced in the below publications:

INFLUENCE OF GASTRIC BYPASS AND SLEEVE GASTRECTOMY ON BLOOD GLUCOSE AND SERUM GIP、GHRELIN OF NORMAL WISTAR RATS.

The effect of Leptin, Ghrelin with Obstractive Sleep Apnea Hypopnea Symptom.


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