E02G0157 Rat Glial Fibrillary Acidic Protein ELISA kit
The Rat Glial Fibrillary Acidic Protein ELISA kit can be used to identify samples from the rat species. Sequestosome can also be called Glial Fibrillary Acidic Protein, GFAP.
Specifications of Rat Glial Fibrillary Acidic Protein ELISA kit
Product Information | |
Cat. No. | E02G0157 |
Product Name | Rat Glial Fibrillary Acidic Protein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GFAP ELISA kit uses an anti-GFAP antibody and an GFAP-HRP conjugate in a competitive enzyme immunoassay method. GFAP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GFAP antibody binding site between GFAP from samples and GFAP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GFAP. Since the number of sites is limited, as more sites are occupied by GFAP from the sample, fewer sites are left to bind GFAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GFAP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Glial Fibrillary Acidic Protein ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GFAP and analogues was observed. | |
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Summary of the Assay Procedure for Rat Glial Fibrillary Acidic Protein ELISA kit
Citations of Rat Glial Fibrillary Acidic Protein ELISA kit
E02G0157 has been referenced in the below publications：
Effects of Early Acupuncture on Serum GFAP and S-100B Protein in Rats with Traumatic Brain Injury.
