E02G0031 Rat Glucagon Like Peptide 1 ELISA kit
The Rat Glucagon Like Peptide 1 ELISA kit can be used to identify samples from the rat species. Glucagon Like Peptide 1 can also be called GCG, Glicentin related polypeptide, GLP 2, Glucagon like peptide 1, Glucagon like peptide 2, Glucagon precursor, GRPP.
Product Information | |
Cat. No. | E02G0031 |
Product Name | Rat Glucagon Like Peptide 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GLP 1 ELISA kit uses an anti-GLP 1 antibody and an GLP 1-HRP conjugate in a competitive enzyme immunoassay method. GLP 1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GLP 1 antibody binding site between GLP 1 from samples and GLP 1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GLP 1. Since the number of sites is limited, as more sites are occupied by GLP 1 from the sample, fewer sites are left to bind GLP 1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLP 1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GLP 1 and analogues was observed. | |
E02G0031 has been referenced in the below publications：
Effect of Sijunzitang on the Level of GLP-1 and Islet Function in Spleen Diabetic Rats.
低频电针“足三里”T2DM大鼠GLP-1分泌影响的实验研究。
Influence of Bariatric Surgery on glucose metabolism of normal rats .
Effect of Liraglutide on improving the glucose metabolism by inhibiting toll-like recpter 4/ toll-like recpter 4 signaling pathway in type 2 diabetes rats.
Clinical Observation and Mechanism of Spleen Qi Treatment of Spleen Deficiency Type 2 Diabetes.
Effects of aerobic exercising combined with glutamine supplement on the serum GLP-1, insulin and blood glucose levels of rats with T2DM.
Related Bluegene Biotech Products
BlueGene Biotech News
