E02G0195 Rat Glucose Transporter 1 ELISA Kit
The Rat Glucose Transporter 1 ELISA Kit can be used to identify samples from the rat species. Glucose Transporter 1 can also be called GLUT1; SLC2A1; solute carrier family 2, facilitated glucose transporter member 1.
Product Information | |
Cat. No. | E02G0195 |
Product Name | Rat Glucose Transporter 1 ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GLUT1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GLUT1. Standards or samples are then added to the microtiter plate wells and GLUT1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GLUT1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GLUT1 are added to each well to “sandwich” the GLUT1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GLUT1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT1 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GLUT1 and analogues was observed. | |
E02G0195 has been referenced in the below publications：
Effects of ischemic preconditioning on expression of VEGF, GLUT1 and BAI1 in focal cerebral infarction rats.
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