E02G0598 Rat Glycine ELISA kit
The Rat Glycine ELISA kit can be used to identify samples from the rat species. Glycine can also be called Gly, Glycine, Glycocol, Glycic acid, Dicarbamic acid.
Specifications of Rat Glycine ELISA kit
Product Information | |
Cat. No. | E02G0598 |
Product Name | Rat Glycine ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ug/ml |
Sensitivity | 1.0 ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD C (0.5mL) | 10 ug/mL | 1 vial |
STANDARD D (0.5mL) | 25 ug/mL | 1 vial |
STANDARD E (0.5mL) | 50 ug/mL | 1 vial |
STANDARD F (0.5mL) | 100 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Gly ELISA kit uses an anti-Gly antibody and an Gly-HRP conjugate in a competitive enzyme immunoassay method. Gly-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Gly antibody binding site between Gly from samples and Gly-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Gly. Since the number of sites is limited, as more sites are occupied by Gly from the sample, fewer sites are left to bind Gly-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Gly concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Glycine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Gly and analogues was observed. | |
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Summary of the Assay Procedure for Rat Glycine ELISA kit
Citations of Rat Glycine ELISA kit
E02G0598 has been referenced in the below publications：
System-wide assembly of pathways and modules hierarchically reveal metabolic mechanism of cerebral ischemia.
