E02G0213 Rat Gonadotropin Releasing Hormone ELISA kit
The Rat Gonadotropin Releasing Hormone ELISA kit can be used to identify samples from the rat species. Gonadotropin Releasing Hormone can also be called Advanced Glycation End Product, AGE.
Specifications of Rat Gonadotropin Releasing Hormone ELISA kit
Product Information | |
Cat. No. | E02G0213 |
Product Name | Rat Gonadotropin Releasing Hormone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GnRH ELISA kit uses an anti-GnRH antibody and an GnRH-HRP conjugate in a competitive enzyme immunoassay method. GnRH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GnRH antibody binding site between GnRH from samples and GnRH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GnRH. Since the number of sites is limited, as more sites are occupied by GnRH from the sample, fewer sites are left to bind GnRH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GnRH concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Gonadotropin Releasing Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GnRH and analogues was observed. | |
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Summary of the Assay Procedure for Rat Gonadotropin Releasing Hormone ELISA kit
Citations of Rat Gonadotropin Releasing Hormone ELISA kit
E02G0213 has been referenced in the below publications：
Effect of the Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi on Sex Hormone Functional Levels in Osteoporosis Rats.
