E02H0022 Rat Heat Shock Protein 70 ELISA kit
The Rat Heat Shock Protein 70 ELISA kit can be used to identify samples from the rat species. Heat Shock Protein 70 can also be called Hsp70, DnaK, 70 Kilodalton Heat Shock Proteins.
Specifications of Rat Heat Shock Protein 70 ELISA kit
Product Information | |
Cat. No. | E02H0022 |
Product Name | Rat Heat Shock Protein 70 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HSP70 ELISA kit uses an anti-HSP70 antibody and an HSP70-HRP conjugate in a competitive enzyme immunoassay method. HSP70-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HSP70 antibody binding site between HSP70 from samples and HSP70-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HSP70. Since the number of sites is limited, as more sites are occupied by HSP70 from the sample, fewer sites are left to bind HSP70-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP70 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Heat Shock Protein 70 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HSP70 and analogues was observed. | |
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Summary of the Assay Procedure for Rat Heat Shock Protein 70 ELISA kit
Citations of Rat Heat Shock Protein 70 ELISA kit
E02H0022 has been referenced in the below publications：
The protective effect and mechanism of alanyl-glutamine dipeptide in treatment of severe acute pancreatitis.
