E02H0237 Rat Histone ELISA kit
The Rat Histone ELISA kit can be used to identify samples from the rat species. Sequestosome can also be called Histone
Specifications of Rat Histone ELISA kit
Product Information | |
Cat. No. | E02H0237 |
Product Name | Rat Histone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Histone ELISA kit uses an anti-Histone antibody and an Histone-HRP conjugate in a competitive enzyme immunoassay method. Histone-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Histone antibody binding site between Histone from samples and Histone-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Histone. Since the number of sites is limited, as more sites are occupied by Histone from the sample, fewer sites are left to bind Histone-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Histone concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Histone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Histone and analogues was observed. | |
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Summary of the Assay Procedure for Rat Histone ELISA kit
Citations of Rat Histone ELISA kit
E02H0237 has been referenced in the below publications：
The Research on the Correlation between Histones and the Experimental Progressing of Sepsis
