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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Homovanillic acid ELISA kit (E02H0326)

E02H0326 Rat Homovanillic acid ELISA kit

The Rat Homovanillic acid ELISA kit can be used to identify samples from the rat species. Homovanillic acid can also be called HVA, 2-(4-Hydroxy-3-methoxyphenyl)acetic acid, 3-Methoxy-4-hydroxyphenylacetic acid, 4-Hydroxy-3-methoxy-benzeneacetic acid.

Products

Specifications of Rat Homovanillic acid ELISA kit

Product Information

Cat. No.

E02H0326

Product Name

Rat Homovanillic acid ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HVA ELISA kit uses an anti-HVA antibody and an HVA-HRP conjugate in a competitive enzyme immunoassay method. HVA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HVA antibody binding site between HVA from samples and HVA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HVA. Since the number of sites is limited, as more sites are occupied by HVA from the sample, fewer sites are left to bind HVA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HVA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Homovanillic acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HVA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Homovanillic acid ELISA kit

Summary of the Assay Procedure for Rat Homovanillic acid ELISA kit

Citations of Rat Homovanillic acid ELISA kit

E02H0326 has been referenced in the below publications:

Effect of Self-made Changpu Yujin Decoction on Monoamine Neurotransmitters and Dopamine System in TS Model Rats.

Ef fects of Xifeng Jingning Decoction on Behavior and Dopamine Nuerotransmltter of Stratium in Rat Model of Tourette Syndrome.

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