E02H0326 Rat Homovanillic acid ELISA kit
The Rat Homovanillic acid ELISA kit can be used to identify samples from the rat species. Homovanillic acid can also be called HVA, 2-(4-Hydroxy-3-methoxyphenyl)acetic acid, 3-Methoxy-4-hydroxyphenylacetic acid, 4-Hydroxy-3-methoxy-benzeneacetic acid.
Product Information | |
Cat. No. | E02H0326 |
Product Name | Rat Homovanillic acid ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HVA ELISA kit uses an anti-HVA antibody and an HVA-HRP conjugate in a competitive enzyme immunoassay method. HVA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HVA antibody binding site between HVA from samples and HVA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HVA. Since the number of sites is limited, as more sites are occupied by HVA from the sample, fewer sites are left to bind HVA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HVA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HVA and analogues was observed. | |
E02H0326 has been referenced in the below publications：
Effect of Self-made Changpu Yujin Decoction on Monoamine Neurotransmitters and Dopamine System in TS Model Rats.
Ef fects of Xifeng Jingning Decoction on Behavior and Dopamine Nuerotransmltter of Stratium in Rat Model of Tourette Syndrome.
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