E02H0014 Rat Hydroxyproline ELISA kit
The Rat Hydroxyproline ELISA kit can be used to identify samples from the rat species. Hydroxyproline can also be called Hyp, L-hydroxyproline;(2S,4R)-4-Hydroxyproline, L-Hydroxyproline, HYP.
Specifications of Rat Hydroxyproline ELISA kit
Product Information | |
Cat. No. | E02H0014 |
Product Name | Rat Hydroxyproline ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ug/ml |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 10 ug/mL | 1 vial |
STANDARD E (0.5mL) | 25 ug/mL | 1 vial |
STANDARD F (0.5mL) | 50 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HYP ELISA kit uses an anti-IHYP antibody and an IHYP-HRP conjugate in a competitive enzyme immunoassay method. IHYP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IHYP antibody binding site between IHYP from samples and IHYP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IHYP. Since the number of sites is limited, as more sites are occupied by IHYP from the sample, fewer sites are left to bind IHYP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IHYP concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Hydroxyproline ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HYP and analogues was observed. | |
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Summary of the Assay Procedure for Rat Hydroxyproline ELISA kit
Citations of Rat Hydroxyproline ELISA kit
E02H0014 has been referenced in the below publications：
Protective effects and mechanism of matrine on high-fructose-diet induced non-alcoholic fatty liver in rats.
