E02I0004 Rat Insulin ELISA kit
The Rat Insulin ELISA kit can be used to identify samples from the rat species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS.
Specifications of Rat Insulin ELISA kit
Product Information | |
Cat. No. | E02I0004 |
Product Name | Rat Insulin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
INS ELISA kit uses an anti-INS antibody and an INS-HRP conjugate in a competitive enzyme immunoassay method. INS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-INS antibody binding site between INS from samples and INS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of INS. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Insulin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between INS and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Insulin ELISA kit
Citations of Rat Insulin ELISA kit
E02I0004 has been referenced in the below publications：
Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats.
Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat.
Preoperative Oral Carbohydrate Improved Postoperative Insulin Resistance in Rats through the PI3K/AKT/mTOR Pathway.
Metformin affects thyroid function in male rats.
Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.
Effect of Sijunzitang on the Level of GLP-1 and Islet Function in Spleen Diabetic Rats.
Protective effects of BDNF on hippocampal neurons under high glucose condition.
Chinese medicine Jinlida ameliorates high-fat dietinduced insulin resistance in rats through alleviating lipid accumulation in skeletal muscle.
GFW Modified act on Polycystic Ovary Syndrome in ExPerimental and Clinical Efficacy of Phlegm and blood stasis type.
The Effect of Acupuncture on Insulin-resistance in Obese Rats.
低频电针“足三里”T2DM大鼠GLP-1分泌影响的实验研究。
Influence of Qingre Jiedu Decoction on TNF-a, IL-1, IL-6 in type 2 diabetic rats.
Influence of Bariatric Surgery on glucose metabolism of normal rats.
The role of exercising in improving the myocardial fibrosis of diabetic rats and its possible mechanism.
Roliram improved cognitive impairment and neuroinflammation in diabetic encephalopathy.
清肝祛湿活血方对非酒精性脂肪肝大鼠胰岛素抵抗的影响。
A study on correlation of cognitive function and expression with beta-amyloid,tumor necrosis factor alpha in hippocampus of diabetic rats.
A study on correlation of cognitive function and expression with beta-amyloid，tumor necrosis factor alpha in hippocampus of diabetic rats.
Dynamic changes of major biomarkers in rats plasma during the development of diabete.
清肝祛湿活血方对非酒精性脂肪肝大鼠ERK1-2磷酸化的影响。
Effect of QizhiJiangtang Capsule on insulin resistance in diabetic rats and its mechanism.
The Study of Melatonin Regulating Lipid Metabolismin Male Rats.
Clinical Observation and Mechanism of Spleen Qi Treatment of Spleen Deficiency Type 2 Diabetes.
Effects of aerobic exercising combined with glutamine supplement on the serum GLP-1, insulin and blood glucose levels of rats with T2DM.
Hypoglycemic Effect of Three Different Extracts from Anoectochilus Genus.
Effects of Endurance Exercise on Hepatic SIRT1 and PGC-1α Protein Expression in Ovariectomized Rats.
Expression of Arc/Arg3.1 in diabetic rat hippocampus and its relationship with cognitive function.
The molecular mechanism of skeletal muscle PGC-1a on insulin resistance induced by high fat diet in rat.
高脂饮食对大鼠骨骼肌脂质中间代谢产物的影响及机制。
Activation of Hippocampal CREB by Rolipram Partially Recovers Balance Between TNF-α and IL-10 Levels and Improves Cognitive Deficits in Diabetic Rats.
Rolipram-induced improvement of cognitive function correlates with changes in hippocampal CREB phosphorylation, BDNF and Arc protein levels.
Effect of melatonin on visceral fat deposition, lipid metabolism and hepatic lipo-metabolic gene expression in male rats.
