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BlueGene Biotech Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit (E02I0090)

E02I0090 Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

The Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit can be used to identify samples from the rat species. Insulin Like Growth Factor Binding Protein 5 can also be called IGFBP5; IBF-5; IBP5; insulin like growth factor binding protein 5.

Products

Specifications of Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Product Information

Cat. No.

E02I0090

Product Name

Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IGFBP5 ELISA kit uses an anti-IGFBP5 antibody and an IGFBP5-HRP conjugate in a competitive enzyme immunoassay method. IGFBP5-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IGFBP5 antibody binding site between IGFBP5 from samples and IGFBP5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IGFBP5. Since the number of sites is limited, as more sites are occupied by IGFBP5 from the sample, fewer sites are left to bind IGFBP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP5 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IGFBP5 and analogues was observed.


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Summary of the Assay Procedure for Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Summary of the Assay Procedure for Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Citations of Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

E02I0090 has been referenced in the below publications:

Short-Term Diet Induced Changes in the Central and Circulating IGF Systems Are Sex Specific.

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