Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit (E02I0090)

BlueGene Biotech Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit (E02I0090)

E02I0090 Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

The Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit can be used to identify samples from the rat species. Insulin Like Growth Factor Binding Protein 5 can also be called IGFBP5; IBF-5; IBP5; insulin like growth factor binding protein 5.

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Specifications of Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Product Information
Cat. No.	E02I0090
Product Name	Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit
Species	Rat
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/mL
Sensitivity	0.1ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

IGFBP5 ELISA kit uses an anti-IGFBP5 antibody and an IGFBP5-HRP conjugate in a competitive enzyme immunoassay method. IGFBP5-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IGFBP5 antibody binding site between IGFBP5 from samples and IGFBP5-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IGFBP5. Since the number of sites is limited, as more sites are occupied by IGFBP5 from the sample, fewer sites are left to bind IGFBP5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP5 concentration in each sample is interpolated from this standard curve.

Quality Control on Rat Insulin Like Growth Factor Binding Protein 5 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between IGFBP5 and analogues was observed.