E02I0392 Rat Irisin ELISA kit
The Rat Irisin ELISA kit can be used to identify samples from the rat species. Irisin can also be called FRCP2, Irisin, Fibronectin type III repeat-containing protein 2.
Specifications of Rat Irisin ELISA kit
Product Information | |
Cat. No. | E02I0392 |
Product Name | Rat Irisin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ug/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ug/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ug/mL | 1 vial |
STANDARD F (0.5mL) | 10 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Irisin ELISA kit uses an anti-Irisin antibody and an Irisin-HRP conjugate in a competitive enzyme immunoassay method. Irisin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Irisin antibody binding site between Irisin from samples and Irisin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Irisin. Since the number of sites is limited, as more sites are occupied by Irisin from the sample, fewer sites are left to bind Irisin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Irisin concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Irisin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Irisin and analogues was observed. | |
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Summary of the Assay Procedure for Rat Irisin ELISA kit
Citations of Rat Irisin ELISA kit
E02I0392 has been referenced in the below publications：
Study on the Function of Irisin in the Metabolism of Polycystic Ovary Syndrome Rat Model.
