E02K0198 Rat keyhole limpet hemocyanin antibody ELISA kit
The Rat keyhole limpet hemocyanin antibody ELISA kit can be used to identify samples from the rat species. keyhole limpet hemocyanin antibody can also be called KLH-IgM, KLH.
Specifications of Rat keyhole limpet hemocyanin antibody ELISA kit
Product Information | |
Cat. No. | E02K0198 |
Product Name | Rat keyhole limpet hemocyanin antibody ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ug/mL |
Sensitivity | 1.0 ug/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 10 ug/mL | 1 vial |
STANDARD C (0.5mL) | 25 ug/mL | 1 vial |
STANDARD D (0.5mL) | 50 ug/mL | 1 vial |
STANDARD E (0.5mL) | 100 ug/mL | 1 vial |
STANDARD F (0.5mL) | 250 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KLH-IgM ELISA kit uses an anti-IKLH-IgM antibody and an IKLH-IgM-HRP conjugate in a competitive enzyme immunoassay method. IKLH-IgM-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IKLH-IgM antibody binding site between IKLH-IgM from samples and IKLH-IgM-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IKLH-IgM. Since the number of sites is limited, as more sites are occupied by IKLH-IgM from the sample, fewer sites are left to bind IKLH-IgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKLH-IgM concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat keyhole limpet hemocyanin antibody ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KLH-IgM and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat keyhole limpet hemocyanin antibody ELISA kit
Citations of Rat keyhole limpet hemocyanin antibody ELISA kit
E02K0198 has been referenced in the below publications：
Evaluation of effect of Shenmai injection on immune function in rats by T cell-dependent antibody response model.
