E02K0027 Rat Klotho ELISA kit
The Rat Klotho ELISA kit can be used to identify samples from the Rat species. Klotho can also be called KLa; Klotho Alpha, KL.
Specifications of Rat Klotho ELISA kit
Product Information | |
Cat. No. | E02K0027 |
Product Name | Rat Klotho ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 pg/ml |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 10 pg/mL | 1 vial |
STANDARD C (0.5mL) | 25 pg/mL | 1 vial |
STANDARD D (0.5mL) | 50 pg/mL | 1 vial |
STANDARD E (0.5mL) | 100 pg/mL | 1 vial |
STANDARD F (0.5mL) | 250 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KL ELISA kit uses an anti-KL antibody and an KL-HRP conjugate in a competitive enzyme immunoassay method. KL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KL antibody binding site between KL from samples and KL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KL. Since the number of sites is limited, as more sites are occupied by KL from the sample, fewer sites are left to bind KL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KL concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Klotho ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Klotho ELISA kit
Citations of Rat Klotho ELISA kit
E02K0027 has been referenced in the below publications：
Expression of Hippocampus Klotho Protein and Insulin-like Growth Factor-1 in Rats with Dementia.
