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BlueGene Biotech Rat Klotho ELISA kit (E02K0027)

E02K0027 Rat Klotho ELISA kit

The Rat Klotho ELISA kit can be used to identify samples from the Rat species. Klotho can also be called KLa; Klotho Alpha, KL.

Products

Specifications of Rat Klotho ELISA kit

Product Information

Cat. No.

E02K0027

Product Name

Rat Klotho ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250 pg/ml

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

10 pg/mL

1 vial

STANDARD C (0.5mL)

25 pg/mL

1 vial

STANDARD D (0.5mL)

50 pg/mL

1 vial

STANDARD E (0.5mL)

100 pg/mL

1 vial

STANDARD F (0.5mL)

250 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

KL ELISA kit uses an anti-KL antibody and an KL-HRP conjugate in a competitive enzyme immunoassay method. KL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KL antibody binding site between KL from samples and KL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KL. Since the number of sites is limited, as more sites are occupied by KL from the sample, fewer sites are left to bind KL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KL concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Klotho ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between KL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Klotho ELISA kit

Summary of the Assay Procedure for Rat Klotho ELISA kit

Citations of Rat Klotho ELISA kit

E02K0027 has been referenced in the below publications:

Expression of Hippocampus Klotho Protein and Insulin-like Growth Factor-1 in Rats with Dementia.


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