E02K0010 Rat Kynurenine ELISA kit
The Rat Kynurenine ELISA kitcan be used to identify samples from the rat species. Kynurenine can also be called KYNU.
Specifications of Rat Kynurenine ELISA kit
Product Information | |
Cat. No. | E02K0010 |
Product Name | Rat Kynurenine ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KYNU ELISA kit uses an anti-IKYNU antibody and an IKYNU-HRP conjugate in a competitive enzyme immunoassay method. IKYNU-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IKYNU antibody binding site between IKYNU from samples and IKYNU-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IKYNU. Since the number of sites is limited, as more sites are occupied by IKYNU from the sample, fewer sites are left to bind IKYNU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKYNU concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Kynurenine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KYNU and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Kynurenine ELISA kit
Citations of Rat Kynurenine ELISA kit
E02K0010 has been referenced in the below publications：
Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression.
