E02L0006 Rat Lactate Dehydrogenase ELISA kit
The Rat Lactate Dehydrogenase ELISA kit can be used to identify samples from the rat species. Lactate Dehydrogenase can also be called EC1.1.1.28, lactate dehydrogenase, L-Lactic Dehydrogenase, (S)-Lactate: NAD+ oxidoreductase,L-LDH, LAD,LD, L-Lactic Dehydrogenase Lactate Dehydrogenase from Rabbit Muscle,
Product Information | |
Cat. No. | E02L0006 |
Product Name | Rat Lactate Dehydrogenase ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LDH ELISA kit uses an anti-LDH antibody and an LDH-HRP conjugate in a competitive enzyme immunoassay method. LDH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LDH antibody binding site between LDH from samples and LDH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LDH. Since the number of sites is limited, as more sites are occupied by LDH from the sample, fewer sites are left to bind LDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LDH concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LDH and analogues was observed. | |
E02L0006 has been referenced in the below publications：
Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury of Hyperlipidemia Rat via Energy Modulation.
Experimental Study On Cardioprotection and Mechanism Of Combined Limb Remote Ischemic Postconditioning and Sevoflurane postcondmoning Against Rat Myocardial Ischemia-Reperfusion Injury and Its mechanism.
Phosphocreatine enhances the protective effects of ischemic postconditioning on myocardial ischaemia-reperfusion injury in rats.
Chaiqinchengqi decoction regulates necrosis-apoptosis via regulating the release of mitochondrial cytochrome c and caspase-3 in rats with acute necrotizing pancreatitis.
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