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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Laminin ELISA kit (E02L0018)

E02L0018 Rat Laminin ELISA kit

The Rat Laminin ELISA kit  can be used to identify samples from the rat species. Laminin can also be called LAM, LN.

Products

Specifications of Rat Laminin ELISA kit

Product Information

Cat. No.

E02L0018

Product Name

Rat Laminin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LN ELISA kit uses an anti-ILN antibody and an ILN-HRP conjugate in a competitive enzyme immunoassay method. ILN-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ILN antibody binding site between ILN from samples and ILN-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ILN. Since the number of sites is limited, as more sites are occupied by ILN from the sample, fewer sites are left to bind ILN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ILN concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Laminin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LN and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Laminin ELISA kit

Summary of the Assay Procedure for Rat Laminin ELISA kit

Citations of Rat Laminin ELISA kit

E02L0018 has been referenced in the below publications:

Studies on functional effect and mechanisms of anti-alcohol and liver-protection of Lingzhisuan Granule.

Protective effects and mechanism of matrine on high-fructose-diet induced non-alcoholic fatty liver in rats.

Comparison of hepatic fibrosis models and associated hepatic fibronectin expression in Wistar rats treated by bile duct ligation and CCl4.


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