En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Leptin ELISA kit (E02L0038)

E02L0038 Rat Leptin ELISA kit

The Rat Leptin ELISA kit can be used to identify samples from the rat species. Leptin can also be called LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, OB, Obese Protein, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS.

Products

Specifications of Rat Leptin ELISA kit

Product Information

Cat. No.

E02L0038

Product Name

Rat Leptin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Leptin ELISA kit uses an anti-Leptin antibody and an Leptin-HRP conjugate in a competitive enzyme immunoassay method. Leptin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Leptin antibody binding site between Leptin from samples and Leptin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Leptin. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Leptin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Leptin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Leptin ELISA kit

Summary of the Assay Procedure for Rat Leptin ELISA kit

Citations of Rat Leptin ELISA kit

E02L0038 has been referenced in the below publications:

Nonalcoholic fatty liver tissue of hepatitis TNFΑ, TGFΒ1, LEPTIN and MAPK expression and drug intervention.

The Effect of Acupuncture on Insulin-resistance in Obese Rats.

Qingrejiedu party of type 2 diabetic rats serum inflammation factors leptin IL resistance element-1 intervention study.

The effect of Leptin, Ghrelin with Obstractive Sleep Apnea Hypopnea Symptom.

The Study of Melatonin Regulating Lipid Metabolismin Male Rats.

Protective effects and mechanism of matrine on high-fructose-diet induced non-alcoholic fatty liver in rats.

Impact of Tangzhiping on Lipid Metabolism and Adipokine in the Rats of Insulin Resistance.

Angiotensin‑converting enzyme‑2 improves diabetic nephropathy by targeting Smad7 for ubiquitin degradation.

Effect of melatonin on visceral fat deposition, lipid metabolism and hepatic lipo-metabolic gene expression in male rats.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.