E02L0021 Rat Luteinizing Hormone ELISA kit
The Rat Luteinizing Hormone ELISA kit can be used to identify samples from the rat species. Luteinizing Hormone can also be called ICSH, Lutropin, Interstitial Cell Stimulating Hormone, LH.
Specifications of Rat Luteinizing Hormone ELISA kit
Product Information | |
Cat. No. | E02L0021 |
Product Name | Rat Luteinizing Hormone ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LH ELISA kit uses an anti-LH antibody and an LH-HRP conjugate in a competitive enzyme immunoassay method. LH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LH antibody binding site between LH from samples and LH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LH. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Luteinizing Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LH and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Luteinizing Hormone ELISA kit
Citations of Rat Luteinizing Hormone ELISA kit
E02L0021 has been referenced in the below publications：
The study of rat polycystic ovarian syndrome(PCOS) model induced by sodium prasterone surfate.
Influences of Maca and Tongkat Ali on The Serum Testosterone Level of Swimming Training Rats.
EXPERIMENTAL STUDY ON BUSHENNING REGULATING RATNEUROTRANSMITTERS AND REPRODUCTIVE FUNCTION IN THE OVULATION INHIBITION OF KIDNEY YANG.
东革阿里对游泳训练大鼠血睾酮水平及运动能力的影响。
Effect of Melatonin Treatment during Pregnancy on Growth of Female Offspring in Rat.
