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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Malondialdehyde ELISA kit (E02M0023)

E02M0023 Rat Malondialdehyde ELISA kit

The Rat Malondialdehyde ELISA kit can be used to identify samples from the rat species. Malondialdehyde can also be called MDA.

Products

Specifications of Rat Malondialdehyde ELISA kit

Product Information

Cat. No.

E02M0023

Product Name

Carcinoembryonic

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

5.0 ng/ml

1 vial

STANDARD C (0.5mL)

10 ng/ml

1 vial

STANDARD D (0.5mL)

25 ng/ml

1 vial

STANDARD E (0.5mL)

50 ng/ml

1 vial

STANDARD F (0.5mL)

100 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MDA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MDA. Standards or samples are then added to the microtiter plate wells and MDA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MDA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MDA are added to each well to “sandwich” the MDA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MDA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MDA concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Malondialdehyde ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MDA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Malondialdehyde ELISA kit

Summary of the Assay Procedure for Rat Malondialdehyde ELISA kit

Citations of Rat Malondialdehyde ELISA kit

E02M0023 has been referenced in the below publications:

Functional status of microvascular vasomotion is impaired in spontaneously hypertensive rat.

Curcumin prevents the non-alcoholic fatty hepatitis via mitochondria protection and apoptosis reduction.

Effect of Huatanzhuyu decoction on MDA, SOD, β-APPmRNA in hippocampus in rat models of Alzheimer’s disease.

Effects of Loaded Swimming Exercise on Knee Osteoarthritis in Rats.

The experimental and clinical Research Based on the Theory of "Xuan Fu" Explore Yizhi Tongxuan Decoction for the Treatment of  Senile Dementia Mechanism.

Liver injury attenuation by curcumin in a rat NASH model: an Nrf2 activation-mediated effect?.

Pancreatic Microcirculation Profiles in the Progression of Hypertension in Spontaneously Hypertensive Rats.

Comparison of Pancreatic Microcirculation Profiles in Spontaneously Hypertensive Rats and Wistar-Kyoto Rats by Laser Doppler and Wavelet Transform Analysis.

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